Specialising in secondary antibodies and conjugates
Jackson ImmunoResearch Laboratories, Inc. specializes in the production and conjugation of affinity-purified secondary antibodies and purified immunoglobulins. Their products are sold primarily to scientists in universities and research institutes throughout the world who are conducting research in the plant, animal, and biomedical sciences.
Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.
The product line includes
FabuLight™ – Fab Labeling primary antibodies with fluorophore-conjugated Fab anti-Fc in Solution Without Compromising Activity
DyLight 405-conjugated secondary antibodies are excited maximally at 400 nm and fluoresce with a peak at 421 nm (Figure 1). They are very bright and photostable when used with confocal microscopes equipped with a 405 nm laser and appropriate emission filter.
Alexa Fluor® 680 and Alexa Fluor® 790 conjugates for Far-red and Infrared Detection on Western Blots
Bovine Anti-Goat IgG (H+L)
Cy2, Cy3, and Cy5 Conjugates are brighter in Plastic mounting Media
Secondary Antibodies for Flow Cytometry
Anti-IgG, Light Chain Specific for Western Blotting after Immunoprecipitation
Anti-Mouse IgG Subclass Specific Antibodies
PerCP Conjugates for FIow Cytometry
Streptavidin a tetrameric bacterial protein isolated from Streptomyces avidinii, has 4 very strong binding sites for biotin. It is similar to egg-white avidin in its affinity for biotin but is used as a replacement for avidin because of its more favorable chemical properties. Unlike avidin which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background.